Scand J Immunol 1989 Oct;30(4):435-40
Capping and co-capping of membrane immunoglobulin and lipid-conjugated
immunoglobulin inserted in the cell membrane of B lymphocytes.
Faro-Rivas J, Clinchy B, Hoiden I, Moller G.
Department of Immunology, University of Stockholm, Sweden.
Human and mouse immunoglobulins (Ig) or F(ab')2 fragments of rabbit Ig were
conjugated to lipids and the conjugates inserted into the membrane of mouse
spleen cells. It was found that nearly all B cells, but not T cells, became
decorated with lipid immunoglobulin. Both endogenous Ig receptors and inserted
Ig capped after the addition of cross-linking F(ab')2 antibodies and in both
cases capping required energy. Capping of endogenous mouse Ig led to co-capping
of inserted human Ig, but the reverse was not true.