E-mail: pdurao at igc.gulbenkian.pt
I am currently doing my second postdoc at the Evolutionary Biology group at IGC under the supervision of Isabel Gordo. I graduated in Biotechnology Engineering in 2002 at the Universidade Lusófona de Humanidades e Tecnologias (ULHT) in Lisbon. After an internship at Pfizer Research & Development (San Diego), I went on and got my PhD on Biochemistry at the Instituto de Tecnologia Química e Biológica (ITQB, Oeiras) where I focused on structure-function studies on the catalytic copper centers of a multicopper oxidase. Afterwards, I did my first postdoc at Max-Planck Institute of Biochemistry (Munich) where I studied the evolutionary effect of folding- and assembly-chaperones on the evolution of the photosynthetic enzyme RuBisCO by applying directed evolution techniques.
Presently, I am interested on the level of epistasis between beneficial mutations in E. coli when interacting with macrophages. I am working on both the intracellular adaptation of E. coli to macrophages and on the effects of antibiotic resistance in this system. I am hoping that my background on biochemistry will bring new insights into the topic and the goal is to complement my approach with the approaches already existing in the lab to understand how we can decrease the frequency of antibiotic resistant bacteria in the context of infections.
Moura de Sousa J, Balbontín R, Durão P, Gordo I (2017) Multidrug-resistant bacteria compensate for the epistasis between resistances. PLOS Biology 15(4): e2001741. https://doi.org/10.1371/journal.pbio.2001741
Durão P, Gülereşi D, Proença J, Gordo I (2016) Enhanced Survival of Rifampin- and Streptomycin-Resistant Escherichia coli Inside Macrophages. Antimicrobial Agents and Chemotherapy 60(7):4324-4332. doi:10.1128/AAC.00624-16.
Durão P, Trindade S, Sousa A & Gordo I (2015) Multiple Resistance at no cost: Rifampicin and Streptomycin a dangerous liaison in the spread of antibiotic resistance. Mol Biol Evol. 32(10):2675-80. (doi: 10.1093/molbev/msv143)
Martins, LO., Durão, P., Brissos, V., Lindley, P., (2015) “Laccases of prokaryotic origin – Enzymes at the interface of protein science and protein technology”. Cell Mol Life 72: 911-922
Durão, P., Aigner, H., Nagy, P., Müller-Cajar, O., Hartl, F.U., Hayer-Hartl, M. (2015) “Opposing effects of Folding and Assembly Chaperones on Evolvability of RuBisCO”. Nat Chem Biol. DOI: 10.1038/nchembio.1715 (2015 February Nat Chem Biol Cover)
Silva, C.S., Durão, P., Fillat, A., Lindley, P.F., Martins, L.O., and Bento, I. (2012) “Crystal structure of the multicopper oxidase from the pathogenic bacterium Campylobacter jejuni CGUG11284: characterization of a metallo-oxidase” Metallomics. 4: 37-47. (2012 January Metallomics Cover)
Chen, Z., Durão, P., Silva, C.S., Pereira, M.M., Todorovic, S., Hildebrandt, P., Bento, I., LindleyP.F., and Martins, L.O. (2010) “The role of Glu498 in the dioxygen reactivity of CotA-laccase from Bacillus subtilis”. Dalton Transactions. 39: 2875-2882.
Durão, P., Chen, Z., Silva, C.S., Soares, C.M., Pereira, M.M., Todorovic, S., Hildebrandt, P., Bento, I., Lindley, P.F., Martins, L.O. (2008), “Proximal mutations at the type 1 copper site of CotA-laccase; spectroscopic, redox, kinetic and structural characterization of I494A and L386A mutants.” Biochem. J. 412: 339-346.
Durão, P., Chen, Z., Fernandes, A.T., Hildebrandt, P., Murgida, D.H., Todorovic, S., Pereira, M.M., Melo, E.P. and Martins, L.O. (2008) “Copper incorporation into recombinant Cota-laccase from Bacillus subtilis – Characterization of Fully Cu-Loaded Enzymes”, J. Biol. Inorg. Chem., 13:183-93.
Melo, E.P., Fernandes, A.T., Durão, P., and Martins, L.O. (2007) “Insight into Stability of Cota-laccase from the spore coat of Bacillus subtilis”, Bio. Chem. Soc. Trans., 35: 1579-82.
Durão, P., Bento, I., Fernandes, A. T., Melo, E. P., Lindley, P. F. and Martins, L.O. (2006) “Perturbations of the T1 copper site in the CotA-laccase from Bacillus subtilis: Structural, Biochemical, Enzymatic and Stability Studies”, J. Biol. Inorg. Chem., 11: 514-26. (2007 JBIC Cover)
Yazbeck, D.R., Durao, P.J., Xie, Z., Tao, J. (2006) “A metal ion-based method for the screening of nitrilases”, Journal of Molecular Catalysis B: Enzymatic, 39, 156-159.
Fernandes, A.R., Durão, P.J., Santos, P.M., Sá-Correia, I. (2003) “Activation and significance of vacuolar H+-ATPase in Saccharomyces cerevisiae adaptation and resistance to the herbicide 2,4-dichlorophenoxyacetic acid”, Biochem Biophys Res Commun. 312:1317-24.